Faculty Detail

Dr Manish Dwivedi

Asst. Professor (Grade-III)

Amity Institute of Biotechnology, Lucknow

Teaching Interests
Biophysics, Structural Biology, Nanotoxicology, Bio-nanotechnology.
Experience
06 Jul 2018 - Present
Asst. Professor (Grade-III),
Amity Institute of Biotechnology, Lucknow
Current Courses Taught
Research Techniques in Biosciences and Biotechnology
Research Interests
Biophysical and Biochemical characterization of membrane proteins (currently Ec-NhaA). Structural and functional studies of membrane proteins. Bio-molecular interactions. Biochemistry, Molecular biology & microbiology. Investigation on membrane proteins as a novel drug targets. Interdisciplinary approach to study protein chemistry.
Publications
Ec-NhaA transporter switches from antagonistic to synergistic antiporting via a single point mutation ,
Nature: Scientific Reports

Asp133 Residue in NhaA Na+/H+ Antiporter Is Required for Stability Cation Binding and Transport ,
Journal of Molecular Biology

Broad phylogenetic analysis of cation/proton antiporters reveals transport determinants ,
Nature Communications

Lysine300 is essential for stability but not for electrogenic transport of the NhaA Na+/H+ Antiporter. ,
The Journal of Biological Chemistry

Replacement of Lys-300 with a glutamine in the NhaA Na+/H+ antiporter of Escherichia coli yields a functional electrogenic transporter. ,
The Journal of Biological Chemistry

Replacement of Lys-300 with a glutamine in the NhaA Na+/H+ antiporter of Escherichia coli yields a functional electrogenic transporter. ,
The Journal of Biological Chemistry

Combustion synthesis and characterization of the Eu doped Y2O3 Nanoparticles. ,
Advanced Science Letters

Evolutionary Inferences on Lysosomal Membrane Proteins (LMPs) defective in metabolic disorders: Cystinosin and Sialin. ,
Advances in Applied Science Research

An evolutionary Account of GPI Anchored Proteins. ,
European Journal of Experimental Biology

A Comparative Genomics based evaluation on Evolutionary aspect of Cystinosin: the Protein defective in Cystinosis, ,
Journal of Computing

In Silico study on Evolutionary account of Lysosomal Associated Membrane Protein -1 (LAMP-1). ,
BVICAM’S International Journal of Information Technology

Overexpression, Isolation, Purification, and Crystallization of NhaA ,
Methods in Enzymology, Membrane Proteins—Engineering, Purification and Crystallization

Nanophosphors-Nanogold immunoconjugates in Isolation of Biomembranes and in Drug Delivery. ,
Nanomedicine for Drug Delivery and Therapeutics (Wiley-Scrivener )

Using Phylogeny to Decipher Electrogenicity in Cation/Proton Antiporters ,
Biophysical journal (Cell Press)
Projects
Biophysical and Biochemical characterization of NhaA Antiporters: structure based study(The Hebrew University of Jerusalem, Israel)
Na+ and H+ are extremely important component of the cells and their specific concentrations are crucial for cells’ survival. Each cell has very efficient tool, Na+/H+ Transporters, to maintain these ions’ concentration. Na+/H+ antiporters are secondary transporters present in the cytoplasmic membrane of all living cells where they play critical roles; pH regulation, maintaining homeostasis of H+ and Na+ ions in the cytoplasm and as a result cell volume. Therefore, these antiporters have long beby (Department of Science & Technology, India)

Biophysical and Biochemical characterization of NhaA Antiporters: structure based study(The Hebrew University of Jerusalem, Israel)
Na+ and H+ are extremely important component of the cells and their specific concentrations are crucial for cells’ survival. Each cell has very efficient tool, Na+/H+ Transporters, to maintain these ions’ concentration. Na+/H+ antiporters are secondary transporters present in the cytoplasmic membrane of all living cells where they play critical roles; pH regulation, maintaining homeostasis of H+ and Na+ ions in the cytoplasm and as a result cell volume.by (Department of Science & Technology, India)
Affiliations
International Academy of Physical Sciences Since 02 Feb 2010

Indian Science Congress Association Since 15 Dec 2008

Israel Chemical Society Since 16 Jun 2015

Israel society for Biochemistry and Molecular biology Since 05 Jan 2016

Proteomics Society (INDIA) Since 11 Dec 2018

The Society of Biological Chemists Since 10 Dec 2018

Indian Biophysical Society Since 12 Dec 2018
Short Description
Dr. Manish Dwivedi has gained M.Sc. (Biotechnology) from C.S.J.M. Kanpur University and he has pursued his Doctorate from University of Allahabad, worked on Lysosomal membrane proteins, subsequently joined the National Research Center for Plant Biotechnology, New Delhi. Dr. Dwivedi was awarded with prestigious PBC postdoctoral fellowship in Israel and got an opportunity to work with Prof. Etana Padan, (pioneer of Ec-Na+/ H+ Antiporter) in Department of Biological Chemistry, The Hebrew University of Jerusalem, Israel and worked in a very close collaboration with Prof. Klaus Fendler (Max-Planck Institute of Biophysics, Frankfurt, Germany), Prof Assaf Friedler (Institute of Chemistry, The Hebrew University of Jerusalem, Israel), Prof Nir Ben-Tal (Tel Aviv University) and Prof. Amnon Horovitz (Department of Structural Biology, Weizmann Institute of Science). Prior to join Amity University, Dr. Dwivedi got awarded with renowned DST-INSPIRE Faculty (2017). Email: mdwivedi@lko.amity.edu

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