Faculty Detail

Dr Rajni Singh

Addl. Director

Amity Institute of Microbial Technology

Qualifications
Graduation : B.Sc. (H) Microbiology -1992,University of Delhi

Post Graduation : M.Sc. (H) Microbiology -1995,G.B. Pant University of Agriculture and Technology,

Doctorate : Ph.D.(Microbilogy)-1999,G.B. Pant University of Agriculture and Technology,
Experience
01 Jun 2006 - Present
Addl. Director,
Amity Institute of Microbial Technology
Current Courses Taught
Research Techniques in Biosciences and Biotechnology
Publications
An extracellular alkaline lipase from an isolate of Enterobacter aerogenes. ,
Springer

Purification of Enterobacter aerogenes plasmid using MnCl2 as compaction agent. ,
Elsevier

Purification of Recombinant Green fluorescent protein by Three Phase Partitioning. ,
Elsevier

Characterization of Metallo-protease produced in Solid State Fermentation by newly isolated Bacillus sp ,
University of Szeged

Microbial Consortium for Effective Degradation of Organic Kitchen Wastes ,
International Association of Computers science & Information technology press

Amylase Production by Solid-State Fermentation of Agro-industrial Wastes Using Bacillus sp. ,
Brazilian society of microbiology

Effect of Activated multi walled carbon nanotubes (MWCNTs) and TiO2 nanoparticles on microbial Structural Integrity. ,
American scientific publisher

Concomitant production of thermostable amylase and protease from Bacillus sp. by solid state fermentation ,
Widner University

Wild apricot (Prunus armeniaca) kernel oil: A strategic alternative to value added fatty acids ,
Essential Oil Resource consultants

Protease production in Solid State Fermentation by Bacillus sp. Journal of Solid Waste Technology & Management ,
Widener University

Biodegradation of BTEX (benzene, toluene, ethyl benzene and xylene) compounds by bacterial strain under aerobic conditions ,
www.journal.ecobiotechnology.com

Statistical optimization of conditions for protease production from Bacillus sp. Acta Biologica Szegediensis ,
University of Szeged

Metal Ion and pH stables production using agro industrial waste ,
www.journal.ecobiotechnology.com

Alkaline Lipase Production from Enterobacter aerogenes by Solid-State Fermentation of Agro-industrial Wastes ,
Inderscience publishers

Thermostable alkaline protease with detergent compatibility from a newly isolated strain of Pseudomonas aeruginosa” ,
indian society of agricultural biochemists

Chemical composition and antimicrobial activity of Walnut Oil ,
Essential oil resource consultants

Simple activity staining protocol for lipases and esterases. ,
Springer

Keratinolytic potential of Bacillus licheniformis RG1: Structural and Biochemical mechanism of feather degradation ,
NRC Research Press

A solvent tolerant isolate of Enterobacter aerogenes. ,
Elsevier

Single step purification of a lipase from Burkholderia multivorans using polypropylene matrix ,
Springer

Antimicrobial Activity of Some Natural Dyes ,
Elsiever

Developments in Diagnosis and Anti-Leishmanial Drugs ,
Hindawi

Bacteriostatic action of fatty acids from Apricot and Walnut oil ,
Indian Journal of Agricultural Biochemistry

Statistical Modeling And Optimization Of Keratinase Production From Newly Isolated Bacillus Subtilis Rse163 ,
International Journal of Advanced Biotechnology and Research.

• Fungi as Builders for Nanomaterials ,
Biotechnology Articles

Novel Metalloprotease Production by Solid State Fermentation of Agroindustrial Residues: A Statistical Approach for Process Optimization ,
Inderscience

. Isolation and identification of xylanolytic enzyme from an effective strain bacillus licheniformis isolated from the decaying wood ,
Indian Res. J. Genet. & Biotech.

Vermicomposting- A Technology for Waste Management and Recycling ,
International Journal of Innovative Horticulture

Hydrolyzing proficiency of keratinases in feather degradation ,
Springer

Biocontrol of Phytopathogenic Fungi using Mycolytic Enzymes Produced by rhizospheric Bacteria of Cicer arietinum ,
The Indian Society of Agricultural Biochemists

Synergistic Effect of Reductase and Keratinase for Facile Synthesis of Protein Coated Gold Nanoparticles ,
The Korean Society for Microbiology and Biotechnology

Contemporaneous Production of Amylase and Protease through CCD Response Surface Methodology by Newly Isolated Bacillus megaterium strain B69. ,
Hindawi

MALDI-TOF MS and CD Spectral analysis for Identification and Structure Prediction of a Purified Novel, Organic Solvent stable, Fibrinolytic Metalloprotease from Bacillus cereus B80. ,
Hindawi

Xylanolytic Enzyme using Agricultural Waste by Solid State Fermentation ,
International Journal of Current Microbiology and Applied Sciences

SIDEROPHORE: STRUCTURAL AND FUNCTIONAL CHARACTERISATION ,
Agriculture (Polnohospodárstvo

MALDI-TOF MS and CD Spectral Analysis for Identification and Structure Prediction of a Purified, Novel, Organic Solvent Stable, Fibrinolytic Metalloprotease from Bacillus cereus B80 ,
BioMed Research International, Hindawi

Microbial Waste Management ,
LAP LAMBERT Academic Publishing

Complete genome sequence of Paracoccus yeei TT13, isolated from human skin. ,
American Society for Microbiology

Molecular modeling of cloned Bacillus subtilis keratinase and its insinuation in Psoriasis treatment using docking studies ,
Indian Journal of Microbiology

Microbial biotransformation: A process for Chemical Alterations. ,
Journal of Bacteriology & Mycology

Concomitant Ability of Siderophore Against Iron Paucity and Fusarium wilt in Lycopersicon esculentum ,
Biosciences Biotechnology Research Asia

Iron acquisition in Maize using Pseudomonas siderophore ,
3 biotech

Enhanced bioactivity of Graphene Oxide-Fe3O4 nanocomposite against pathogenic bacterial strains ,
Journal of Nano Medicine

Synthesis, Characterization and Antibacterial Activity of Zn (II) Tetraaza Macrocyclic Complexes ,
Global Journal for Research Analysis

Novel Bacillus Consortium for Degradation of 2,4- Dinitrotoluene: A Xenobiotic Compound ,
British Microbiology Research Journal

Phylogenetical coherence of Pseudomonas in unexplored soils of Himalayan region ,
3 biotech

Cloning of merA gene from Methylotenera mobilis for mercury biotransformation ,
Indian Journal of Microbiology

Surface modified alumina compact: a potential material for decontamination of trivalent and hexavalent chromium and growth inhibitor of microbes from water ,
Advanced Materials letters

Environmentally Benign TiO2 nanomaterials for removal of Heavy Metal Ions with interfering ions present in Tap Water ,
materials today proceedings

Effect of pseudomonas on micronutrient status of forest and agricultural soil of uttarakhand ,
Ecology, Environment and conservation

Purification and characterization of a Bacillus subtilis keratinase and its prospective application in feed industry ,
Acta Biologica Szegediensis

Phylogenetic analysis, optimization of nutitional media composition and immobilization of xylanase enzyme ,
International Research Journal of Pharmacy

Macrophomina phaseolina: microbased biorefinery for gold nanoparticle production ,
Springer

Flower Shaped Gold Nanoparticles: Biogenic Synthesis Strategies and Characterization ,
Springer

Buffering Reduces Potassium Solubilising Potential of Selected Bacterial Isolates

Psychrotrophs of the genus Janthinobacterium with potential to weather potassium aluminosilicate mineral ,
Springer

Journal of Pure and Applied Microbiology

Bioconversion of Hg and Molecular Characterization of merA and merB Gene in a Clone Constructed by Culture Independent Technique

Microwave Mediated Impregnation of Silver Nanoparticles on Silk Fabric for Hygienic Clothing ,
Med Crave

Ciprofloxacin Functionalized Biogenic Gold Nanoflowers as nanoantibiotics against pathogenic bacterial strains
Projects
Effective decomposition of Organic waste in Military areas with emphasis on kitchen waste using Micr(DRL, Tezpur)
The major objective of the project was to use the microbial consortium available and rediscover more microorganisms that can be used for the safe decomposition of the wastes, kitchen scraps, as well as that yields byproducts that can be used in agriculture, horticulture, organic farming, etc. We have different microbial strains (40 protease, 35 amylase, 52 lipase, 37 FPase and CMCase, 34 xylanase producers) having capabilities to degrade protein, oil and fats, amylose, cellulose and hemicellulby (DRDO)

ARDRA based simple identification protocol for distinguishing Bacillus and Bacillus related genera.(DRDE, Gwalior)
This project is to explore the extremely rich microbial diversity of the Indian subcontinent with an aim to develop molecular probes based on Amplified ribosomal DNA restriction analysis (ARDRA) for identification of Bacillus and Bacillus-related genera amongst the various Gram positive spore formers used industrially. by (DRDE)

Application of Micro-organisms for biodegradation of explosives & related compounds(CFEES, DRDO)
To develop a process of decontaminating the nitro-substituted compounds TNT, HMX and RDX contaminants by converting the contaminants into harmless materials thereby decontaminating the soil to either partial decontamination or complete remediation. In our lab we have formulated more than ten consortiums with promising explosive degradation capabilities. Introduction of allochthonous microorganisms in natural environment for the purpose of increasing the rate or extent or both of degradation of by (DRDO)

Feather Waste Management using Microbial Enzymes(MoEF)
Feather waste is utilized on a limited basis as a dietary protein supplement for animal feedstuffs. These processes, in addition to being energy intensive, results in the loss of some essential amino acids. Moreover, the feather meal thus produced has been found to be poorly digestible by animals. This project is very important to improve feather digestibility by microbial proteolytic systems for hydrolysis of feather to soluble proteins and amino acids. It offers a cheap and mild reaction condby (MoEF)

Hypervariant 16S rDNA Sequence as a Proposed Molecular Tag for Grouping and Identification of Baci(Nil)
simple protoclos for Grouping of industrially important Bacillus speciesby (DST-SERB)
Affiliations
Indian Society of Agricultural Biochemists Since 31 Mar 2012

International Association of Computer Science and Information Technology Since 11 Mar 2010
Activities
• Listed in Morquis Who’s Who list in 2011

• Listed in “2000 outstanding intellectuals of the 21st century”, published in Britain.

• Best poster presentation award on a paper entitled “Lipase production from Enterbacter sp. by solid state fermentation” National Symposium on Environmental Biology. Saurashtra University, Rajkot, India

• Recipient of University Fellowship from G. B. Pant University of Agriculture and Technology, Pantnagar, Nainital, India (from 1995-1997)

• Recipient of Senior Research Fellowship from Council of Scientific and Industrial Research (CSIR), Govt. of India (1997-2000)

• Recipient of DST Travel grant for attending the conference “Feather Waste Management Using Microbial Keratinase,” on Solid Waste Technology and Management.2009: 15-18 March, Widener University, Philadelphia, USA.

• Recipient of INSA travel fellowship for oral presentation “Concomitant Production of Thermostable Amylase and Protease from Bacillus sp. By Solid State Fermentation” in the 25th International Conference on Solid Waste Technology and Management.2010: 14-17 March, Widener University, Philadelphia, USA.

• Recipient of DST Travel grant for oral presentation “Efficacy of Microbes for Organic waste Management” in 12th International Conference on Environment Science and Technology at Greece during 8-10 Sep. 2011

• Listed in “2000 outstanding intellectuals of the 21st century”, published in Britain
Patents
A method for the detection of chemical adulterants in synthetic milk and Ready-To-Use Kit for the Detection of Synthetic Milk. ,DElhi,India - Complete ( 711/DEL/2004 )

Method and kit to check the oxidation of edible oil. ,noida,India - Complete ( 814/DEL/2009 )

Rapid Downstream Processing of Proteases and Lipases for Application in Detergent Industry ,Noida,India - Complete ( 1336/DEL/2009 )

Easy distinction of Bacillus cereus Group Amongst the ,Noida,India - Complete ( 2656/DEL/2008 )

Herbal dry composition for cleaning and moisturizing purpose. ,delhi,India - Complete ( 1239/DEL/2011 )

Biological Stabilization and Mineralization of Organic Kitchen waste by Microbial Consortium. ,Delhi,India - Complete ( 1240/DEL/2011 )

Microwave mediated impregnation of silver nanoparticles on fabrics for hygienic clothing ,Delhi,India - Complete ( 2805/DEL/2011 )

Unique Solvent stable Mn2+ activated Metalloprotease from Bacillus sp. ,Delhi,India - Complete ( 3362/DEL/2011 )

Herbal cream with natural antioxidants for wounds and burns. ,Delhi,India - Complete ( 353/DEL/2012 )

Synthesis of gold nanoparticles from phytopathogenic fungus Macrophomina phasolina. ,Delhi,India - Complete ( 1551/DEL/2012 )

Novel application of keratinase from a newly isolated Bacillus subtilis RSE163 for synthesis of spherical shaped Gold nanoparticles ,Delhi,India - Complete ( 2323/DEL/2012 )

Synergistic composition of apricot and walnut oil as novel broad spectrum anti-microbial. ,Delhi,India - Complete ( 3862/DEL/2012 )
Conferences attended
25th International Conference on Solid Waste Technology and Management.
Organized By :Widener University,Philadelphia, USA.

24th International Conference on Solid Waste Technology and Management
Organized By :Widener University, Philadelphia, USA

International Conference on Biotechnology and Food Science
Organized By :• International Association of Computer Science and Information Technology (IACSIT)

International Conference on Role of Biomolecules in Food Security and Health Improvement,
Organized By :Indian Society of Agricultural Biochemists (ISAB)

12th international conference on Environmental Science and Technology,
Organized By :Global nest

50th Annual Conference of AMI
Organized By :AMI

102 Indian Science Congress
Organized By :Mumbai University

101 Indian Science Congress
Organized By :Jammu University

the International Conference on Translational Nanomedicine (T-NANO 2014)
Organized By :the Institute of Life Sciences (ILS), Ahmedabad University

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